A Secret Weapon For attune flow cytometer
A Secret Weapon For attune flow cytometer
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Knowledge-enriched characterization of mobile populations. Morphological info from photographs can add to the richness of flow cytometry info. Such as, the figure displays an usually standard apoptosis assay using Annexin V and PI, adding mobile imaging to characterize cells in Each individual population to reveal morphologically unique features. These insights could not happen to be received from multiplex staining alone.
As samples are obtained over the Attune CytPix Flow Cytometer, the higher-pace brightfield camera captures and merchants photos of detected activities in a amount as many as 6,000 visuals per 2nd, dependant upon the flow rate and image measurement. For increased adaptability, Attune Cytometric Computer software enables you to modify the graphic seize frequency as essential and select a specific gate for graphic capture, if expected.
(B) Uncooked 264.7 macrophages will often be annotated inaccurately with regard to particle depend because of their complexity and noncircular borders. The quantity of singlets available for Examination raises after the consumer trains and employs a far more exact model.
Discover new insights into your experimental data by visualizing populations employing combined fluorescence and morphology knowledge. Our program interface wizards help downsampling, concatenation (merged documents) and dimensionality resources which can be crafted into the software package for an intuitive, streamlined info Assessment working experience.
"A picture isn't really fairly really worth 1000 dots, but it really helps to see pictures. It is excellent to determine photographs that correlate with staining. For instance, AnnexinV-favourable cells which might be morphologically various than healthier cells, or CD14+ monocytes which are more substantial and more textured than CD3+/CD4+/CD14- lymphocytes.
Right here, a highly skilled person has gated singlets confidently. Following analyzing the handbook singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate is made up of in excess of 4% aggregates.
Pair your Attune flow cytometer with the autosampler for increased workflow effectiveness, and increase robotic integration for maximum throughput.
Steady impression high-quality even at superior throughput Acoustic focusing in addition to a large-velocity camera Merge to impression these T cells continuously at very low or high flow costs. Easily change concentration and digital camera settings to fulfill experimental requirements.
The Attune NxT cytometer is definitely the trustworthy workhorse instrument your lab can depend upon for consistent and trustworthy overall performance. The Attune CytPix design includes a significant-pace brightfield camera and AI-driven impression Evaluation software, with the latest upgrades including machine Understanding instruments and an intuitive consumer interface, enabling Improved details precision, precision, and more quickly insights into sample biology.
Users can look at celebration pictures in true-time to substantiate sample excellent, optimize protocols, and history the morphology of every mobile populace determined for Investigation. Imaging capture also Gains from acoustic focusing, which helps to position the cells making sure that a pointy, centered graphic is obtained.
Sound waves enable specific mobile alignment, causing Remarkable information quality and ~10X speedier sample flow costs.
We previously demonstrated the strength of imaging CAR-T/Ramos mobile interactions. Enable’s examine just the population of greatest curiosity, the double optimistic occasions, to learn more. We can now use extended morphological parameters (here, circularity and skewness of depth) to even more examine the characteristics of such populations and refine gating on these gatherings, increasing details robustness.
Built-in dimensionality reduction instruments amplified resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to confirm predicted inhabitants resolution in lysed complete human blood. Consumer can view situations from Just about every cluster for visual affirmation of cell phenotypes.
No clean/no lyse leukocyte analysis with imaging. Cells were being acquired from 24-hour-outdated blood by dilution in one mM EDTA ( Qualified UX/UI allows end users to practice AI products for expanded applications. (A) The “Practice Product” feature presents annotation resources and authentic-time design instruction precision.
Gating on these extended parameters permits you to rapidly and properly recognize populations of interest and ensure gating strategy with little or no manual assessment. The Attune software package provides use of point out-of-the-art machine Finding out Assessment equipment in just an expertly made user interface, rendering it exceptionally simple more info to operate.
As the mobile line had Formerly been analyzed within the Attune CytPix Flow Cytometer, the researchers went again to the photographs and were being capable to document the microbial infection not less than 5 times previously.
The Attune CytPix Flow Cytometer lets consumers to visualize samples for the duration of acquisition, confirm the accuracy of gating approaches, and assist outcomes with dependable documentation for publication functions.
What appeared to be a homogeneous populace is in fact more assorted—an Perception that should be regarded as when interpreting results.